HPLC-Based Automated Synthesis and Purification of Carbohydrates.
Gustavo A KashiwagiLaura PetrosilliSamira EscopyLuigi LayKeith J StineCristina De MeoAlexei V DemchenkoPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2024)
Reported herein is a new HPLC-based automated synthesizer (HPLC-A) capable of a temperature-controlled synthesis and purification of carbohydrates. The developed platform allows to perform various protecting group manipulations as well as the synthesis of O- and N-glycosides. A fully automated synthesis and purification was showcased in application to different carbohydrate derivatives including glycosides, oligosaccharides, glycopeptides, glycolipids, and nucleosides.