Assay performance and stability of uracil and dihydrouracil in clinical practice.

Jonathan E KnikmanHilde RosingHenk-Jan GuchelaarAnnemieke CatsJos H Beijnen

Published in: Cancer chemotherapy and pharmacology (2023)

A maximum of 1 h at RT between sampling and processing is recommended to ensure reliable U and DHU results. Assay performance tests showed that our UPLC-MS/MS method was robust and reliable. Additionally, we provided a guideline for proper sample handling, processing and reliable quantification of U and DHU.

Keyphrases

ms ms
clinical practice
high throughput
simultaneous determination
liquid chromatography tandem mass spectrometry
high resolution
tandem mass spectrometry
ultra high performance liquid chromatography