Instrumentation for xPCR Incorporating qPCR and HRMA.

A microfluidic PCR device was developed that enables DNA amplification at speeds as fast as 2 s/cycle, with concurrent detection and amplification. Two targets were amplified from human genomic DNA. By observing the fluorescence emitted by a DNA dye while the sample is amplified, it is possible to obtain both qPCR and spatial melting information about the amplified product. The speed and integration of the device make it conducive to while-you-wait diagnostic tests that do not require post-PCR analysis.