Clusterin-carrying extracellular vesicles derived from human umbilical cord mesenchymal stem cells restore the ovarian function of premature ovarian failure mice through activating the PI3K/AKT pathway.
Jing HeChunchun AoMao LiTaoran DengShuo ZhengKe ZhangChengshu TuYu OuyangRuibo LangYijia JiangYifan YangChangyong LiDongcheng WuPublished in: Stem cell research & therapy (2024)
Administration of UC-MSCs and UC-MSCs-derived EVs on day 6 of CTX/BUS injection could effectively improve the ovarian function of POF mice. UC-MSCs-derived EVs carrying CLU promoted proliferation and inhibited apoptosis of ovarian granulosa cells through activating the PI3K/AKT pathway. This study identifies a previously unrecognized molecular mechanism of UC-MSCs-mediated protective effects on POF, which pave the way for the use of cell-free therapeutic approach for POF.
Keyphrases
- mesenchymal stem cells
- umbilical cord
- cell free
- signaling pathway
- cell cycle arrest
- bone marrow
- induced apoptosis
- cell therapy
- endothelial cells
- high fat diet induced
- oxidative stress
- endoplasmic reticulum stress
- cell death
- genome wide
- stem cells
- metabolic syndrome
- type diabetes
- gene expression
- cell proliferation
- pi k akt
- insulin resistance
- dna methylation
- multidrug resistant
- klebsiella pneumoniae
- ultrasound guided
- escherichia coli