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Identifying pneumococci in parapneumonic pleural effusion: Is there a role for culture-independent methods?

Kauana PizzuttiVinícius P PerezCaroline BarbieroPedro A d'AzevedoGilberto B FischerCícero Armídio Gomes Dias
Published in: Pediatric pulmonology (2019)
The reduced sensitivity of culture for pneumococcal detection can be improved through the addition of IMT and qPCR analysis. The utility of qPCR combining detection of lytA and a marker of β-lactam susceptibility should be explored further.
Keyphrases
  • loop mediated isothermal amplification
  • real time pcr
  • label free
  • multidrug resistant
  • data analysis