A New Preclinical Decision Support System Based on PET Radiomics: A Preliminary Study on the Evaluation of an Innovative 64 Cu-Labeled Chelator in Mouse Models.
Viviana BenfanteAlessandro StefanoAlbert ComelliPaolo GiacconeFrancesco Paolo CammarataSelene RichiusaFabrizio ScopellitiMarco PomettiMilene FicarraSebastiano CosentinoMarcello LunardonFrancesca MastrottoAlberto AndrighettoAntonino TuttolomondoRosalba ParentiMassimo IppolitoGiorgio Ivan RussoPublished in: Journal of imaging (2022)
The 64 Cu-labeled chelator was analyzed in vivo by positron emission tomography (PET) imaging to evaluate its biodistribution in a murine model at different acquisition times. For this purpose, nine 6-week-old female Balb/C nude strain mice underwent micro-PET imaging at three different time points after 64 Cu-labeled chelator injection. Specifically, the mice were divided into group 1 (acquisition 1 h after [ 64 Cu] chelator administration, n = 3 mice), group 2 (acquisition 4 h after [ 64 Cu]chelator administration, n = 3 mice), and group 3 (acquisition 24 h after [ 64 Cu] chelator administration, n = 3 mice). Successively, all PET studies were segmented by means of registration with a standard template space (3D whole-body Digimouse atlas), and 108 radiomics features were extracted from seven organs (namely, heart, bladder, stomach, liver, spleen, kidney, and lung) to investigate possible changes over time in [ 64 Cu]chelator biodistribution. The one-way analysis of variance and post hoc Tukey Honestly Significant Difference test revealed that, while heart, stomach, spleen, kidney, and lung districts showed a very low percentage of radiomics features with significant variations ( p -value < 0.05) among the three groups of mice, a large number of features (greater than 60% and 50%, respectively) that varied significantly between groups were observed in bladder and liver, indicating a different in vivo uptake of the 64 Cu-labeled chelator over time. The proposed methodology may improve the method of calculating the [ 64 Cu]chelator biodistribution and open the way towards a decision support system in the field of new radiopharmaceuticals used in preclinical imaging trials.
Keyphrases
- pet imaging
- positron emission tomography
- high fat diet induced
- computed tomography
- aqueous solution
- metal organic framework
- stem cells
- clinical trial
- squamous cell carcinoma
- pet ct
- high resolution
- minimally invasive
- cell therapy
- mouse model
- contrast enhanced
- mass spectrometry
- bone marrow
- atrial fibrillation
- skeletal muscle
- case control