Detection of cellular senescence is important not only in the study of senescence in various biological systems, but also in various practical applications such as image-guided surgical removal of senescent cells, as well as the monitoring of drug-responsiveness during cancer therapies. Due to the lack of suitable imaging probes for senescence detection, particularly in living subjects, we have developed an activatable near-infrared (NIR) molecular probe with far-red excitation, NIR emission, and high "turn-on" ratio upon senescence-associated β-galactosidase (SABG) activation. We present here the first successful demonstration of NIR imaging of DNA damage-induced senescence both in vitro and in human tumor xenograft models.
Keyphrases
- dna damage
- endothelial cells
- fluorescence imaging
- dna repair
- fluorescent probe
- oxidative stress
- high resolution
- living cells
- photodynamic therapy
- high glucose
- stress induced
- induced apoptosis
- diabetic rats
- small molecule
- label free
- papillary thyroid
- single molecule
- drug delivery
- mass spectrometry
- drug induced
- cell proliferation
- sensitive detection
- endoplasmic reticulum stress
- cell death
- squamous cell
- adverse drug