Portable light-sheet optofluidic microscopy for 3D fluorescence imaging flow cytometry.
Jeonghwan SonBiagio MandracchiaAaron D Silva TrenkleGabriel A KwongShu JiaPublished in: Lab on a chip (2023)
Imaging flow cytometry (IFC) combines conventional flow cytometry with optical microscopy, allowing for high-throughput, multi-parameter screening of single-cell specimens with morphological and spatial information. However, current 3D IFC systems are limited by instrumental complexity and incompatibility with available microfluidic devices or operations. Here, we report portable light-sheet optofluidic microscopy (PLSOM) for 3D fluorescence cytometric imaging. PLSOM exploits a compact, open-top light-sheet configuration compatible with commonly adopted microfluidic chips. The system offers a subcellular resolution (2-4 μm) in all three dimensions, high throughput (∼1000 cells per s), and portability (30 cm ( l ) × 10 cm ( w ) × 26 cm ( h )). We demonstrated PLSOM for 3D IFC using various phantom and cell systems. The low-cost and custom-built architecture of PLSOM permits easy adaptability and dissemination for broad 3D flow cytometric investigations.
Keyphrases
- flow cytometry
- high throughput
- single cell
- low cost
- fluorescence imaging
- high resolution
- rna seq
- single molecule
- photodynamic therapy
- induced apoptosis
- high speed
- magnetic resonance imaging
- cell cycle arrest
- minimally invasive
- cell therapy
- oxidative stress
- computed tomography
- health information
- signaling pathway
- label free
- cell death
- bone marrow
- circulating tumor cells
- quantum dots