A dual-labeling fluorescent probe to track lysosomal polarity and endoplasmic reticulum dynamics during ferroptosis.

A polarity-sensitive probe was developed to simultaneously label lysosomes and endoplasmic reticulum (ER) via its dansylamide and rhodamine fluorescence, respectively, enabling ratiometric polarity detection and stable dual-labeling. The fragmented ER network and increased lysosomal polarity during ferroptosis were revealed, which facilitates the understanding of ferroptotic mechanisms.