Pyrococcus furiosus Argonaute-mediated nucleic acid detection.

Lixin MaLongyu WangFei WangWenqiang LiYang LiuAitao LiYuan WangWuxiang MaoChao ZhaiLixin Ma

Published in: Chemical communications (Cambridge, England) (2019)

We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.

Keyphrases

nucleic acid
loop mediated isothermal amplification
real time pcr
label free
single molecule
single cell
dna binding
transcription factor
circulating tumor cells