Statistical indices of masculinity-femininity (M-F) summarize multivariate profiles of sex-related traits as positions on a single continuum of individual differences, from masculine to feminine. This approach goes back to the early days of sex differences research; however, a systematic discussion of alternative M-F indices (including their meaning, their mutual relations, and their psychometric properties) has been lacking. In this paper I present an integrative theoretical framework for the statistical assessment of masculinity-femininity, and provide practical guidance to researchers who wish to apply these methods to their data. I describe four basic types of M-F indices: sex-directionality, sex-typicality, sex-probability, and sex-centrality. I examine their similarities and differences in detail, and consider alternative ways of computing them. Next, I discuss the impact of measurement error on the validity of these indices, and outline some potential remedies. Finally, I illustrate the concepts presented in the paper with a selection of real-world datasets on body morphology, brain morphology, and personality. An R function is available to easily calculate multiple M-F indices from empirical data (with or without correction for measurement error) and draw summary plots of their individual and joint distributions.