Biochemical characterization of OXA-244, an emerging OXA-48 variant with reduced β-lactam hydrolytic activity.

Our data confirm that the R214G mutation (present in OXA-244) results in reduced carbapenem- and temocillin-hydrolysing activity, confirming the crucial role of residue 214 in the hydrolysis of these substrates by OXA-48-like β-lactamases.