Determination of Sulfonamides in Chicken Muscle by Pulsed Direct Current Electrospray Ionization Tandem Mass Spectrometry.
Xian FuHengxing LiangBing XiaChunyan HuangBaocheng JiYan ZhouPublished in: Journal of agricultural and food chemistry (2017)
A simple and rapid approach for the simultaneous detection of trace amounts of six sulfonamides in chicken muscle was developed using pulsed direct current electrospray ionization tandem mass spectrometry (pulsed-dc ESI-MS/MS). The pretreatment of chicken muscle samples consisted of two steps: acetonitrile extraction and n-hexane delipidation. Sulfonamides do not need to be derivatized or chromatographed prior to pulsed-dc ESI-MS/MS. The factors affecting the performance of pulsed-dc ESI-MS/MS were studied. Under optimum conditions, the quantitative performance of pulsed-dc ESI-MS/MS was validated according to European Union Decision 2002/657/EC, and the sensitivity of pulsed-dc ESI-MS/MS was 3 times higher than that of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The limits of detection obtained by pulsed-dc ESI-MS/MS were in the range of 0.07-0.11 μg/kg. The proposed method was simple, rapid, and sensitive, and was successfully used for quantitation and rapid screening of sulfonamides in real chicken muscle samples.
Keyphrases
- ms ms
- liquid chromatography tandem mass spectrometry
- ultra high performance liquid chromatography
- solid phase extraction
- tandem mass spectrometry
- high performance liquid chromatography
- simultaneous determination
- dendritic cells
- liquid chromatography
- loop mediated isothermal amplification
- molecularly imprinted
- skeletal muscle
- gas chromatography
- high resolution
- mass spectrometry
- heavy metals
- risk assessment
- decision making