Efficient Separation of C-Tetramethylcalix[4]resorcinarene Conformers by Means of Reversed-Phase Solid-Phase Extraction.
Héctor Manuel Pineda-CastañedaMauricio MaldonadoZuly Jenny RiveraPublished in: ACS omega (2022)
A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to study the conformer formation generated during the reaction for obtaining C -tetramethylcalix[4]resorcinarene. The chromatographic method was used to design a strategy for purifying the reaction products, using solid-phase extraction columns (RP-SPE) and gradient elution. The chromatographic profiles of the cyclocondensation reaction between resorcinol and acetaldehyde show the presence of three products under the different reaction and precipitation conditions studied. Using RP-SPE, it was possible to enrich the products, which were later characterized by means of RP-HPLC and 1 H nuclear magnetic resonance (NMR). This investigation explored and established a new method for RP-HPLC analysis and RP-SPE separation of conformational isomers obtained in the formation reaction of C -tetramethylcalix[4]resorcinarene.
Keyphrases
- solid phase extraction
- high performance liquid chromatography
- simultaneous determination
- liquid chromatography
- tandem mass spectrometry
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- magnetic resonance
- gas chromatography mass spectrometry
- mass spectrometry
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- gas chromatography
- ms ms
- electron transfer
- high resolution
- computed tomography
- molecular dynamics simulations
- single molecule
- solid state