Ru(III)-Based Metal-Organic Gels: Intrinsic Horseradish and NADH Peroxidase-Mimicking Nanozyme.

Highly active, stable, and cost-effective enzyme-mimicking nanomaterials (nanozymes) hold the potential to be an alternative to replace natural enzymes for the catalysis of enzyme-like reactions in various applications. Here, novel 3D ruthenium-based metal-organic gels (Ru-MOGs) with fibrillar network structures have been successfully synthesized using a facile one-step strategy at room temperature. Surprisingly, the developed 3D fibrillar networked Ru-MOGs simultaneously possess intrinsic horseradish peroxidase and NADH peroxidase mimetic activities. Meanwhile, the horseradish peroxidase mimetic catalytic activity displays well in both acidic environment and alkaline condition. Kinetic analysis reveals that Ru-MOGs make an effective peroxidase mimic with exceptionally high catalytic velocity (Vm), substrate binding affinity (Km), and catalytic efficiency (Kcat/Km). Furthermore, as a proof-of-concept, the mimetic enzyme property of this material was further used to establish a chemiluminescent biosensing platform for glucose detection. These easily synthesized Ru-MOGs as highly active and novel nanozymes not only suggests a bright future for the nanomaterials as enzyme mimics but also provides new insights into the properties of MOGs, greatly broadening and advancing their applications in biocatalysis and bioassays.