Development and standardization of a specific real-time PCR assay for the rapid detection of Candida auris.
Ahmad IbrahimSophie Alexandra BaronHanane YousfiLinda HadjadjRym LalaouiSerge MorandJean-Marc RolainFadi BittarPublished in: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology (2021)
Candida auris is an emerging multiresistant pathogen causing nosocomial fungal infection. Specific detection and identification are necessary. Our goal is to develop a new qPCR system that enables rapid detection of C. auris, based on a GPI (glycosyl-phosphatidylinositol) protein-encoding gene. This system is reproducible and sensitive with a limit of detection of 13 C. auris CFU/qPCR reaction. The 100% specificity of this system is confirmed on 2073 clinical and environmental samples, 50 different bacterial species, and 9 Candida spp. (70 strains). This system is suitable to correctly identify C. auris infections and to trace its source.