Utilization of cytology smears improves success rates of RNA-based next-generation sequencing gene fusion assays for clinically relevant predictive biomarkers.

Nisha S RamaniHui ChenRussell R BroaddusAlexander J LazarRajyalakshmi LuthraL Jeffrey MedeirosKeyur P PatelAsif RashidMark J RoutbortJohn StewartZhenya TangRoland BassettJawad ManekiaBedia A BarkohHyvan DangSinchita Roy-Chowdhuri

Published in: Cancer cytopathology (2020)

The success of RNA-based NGS fusion testing depends on the quality and quantity of RNA extracted. The use of direct smears significantly improves the adequacy of cytologic samples for RNA fusion testing for predictive biomarkers.

Keyphrases

nucleic acid
copy number
high throughput
genome wide
gene expression
high grade
dna methylation
single cell