Contextualizing protein representations using deep learning on protein networks and single-cell data.

Understanding protein function and discovering molecular therapies require deciphering the cell types in which proteins act as well as the interactions between proteins. However, modeling protein interactions across diverse biological contexts, such as tissues and cell types, remains a significant challenge for existing algorithms. We introduce P innacle , a flexible geometric deep learning approach that is trained on contextualized protein interaction networks to generate context-aware protein representations. Leveraging a human multiorgan single-cell transcriptomic atlas, P innacle provides 394,760 protein representations split across 156 cell type contexts from 24 tissues and organs. P innacle 's contextualized representations of proteins reflect cellular and tissue organization and P innacle 's tissue representations enable zero-shot retrieval of the tissue hierarchy. Pretrained P innacle protein representations can be adapted for downstream tasks: to enhance 3D structure-based protein representations (PD-1/PD-L1 and B7-1/CTLA-4) at cellular resolution and to study the genomic effects of drugs across cellular contexts. P innacle outperforms state-of-the-art, yet context-free, models in nominating therapeutic targets for rheumatoid arthritis and inflammatory bowel diseases, and can pinpoint cell type contexts that are more predictive of therapeutic targets than context-free models (29 out of 156 cell types in rheumatoid arthritis; 13 out of 152 cell types in inflammatory bowel diseases). P innacle is a network-based contextual AI model that dynamically adjusts its outputs based on biological contexts in which it operates.