A novel infective endocarditis virulence factor related to multiple functions for bacterial survival in blood was discovered in Streptococcus sanguinis .
Vysakh AnandanLiang BaoZan ZhuJennifer BradleyValery-Francine AssiHenna ChavdaTodd KittenPing XuPublished in: bioRxiv : the preprint server for biology (2024)
We identified the role of a conserved hypothetical protein (SSA_0451) in S. sanguinis that is involved in the virulence of infective endocarditis. An in vitro whole blood killing assay and rabbit endocarditis model studies revealed that the SSA_0451 mutant (ΔSSA_0451) was significantly less virulent than the wild-type (SK36) and its complementation mutant (ΔSSA_0451C). The mechanism underlying the SSA_0451 mutant's reduced virulence in infective endocarditis was evidentially linked to oxidative stress and environmental stress. The genes related to the survival of S. sanguinis in an oxidative stress environment were downregulated in ΔSSA_0451, which affected its survival in blood. Our findings suggest that SSA_0451 is a novel IE virulence factor and a new target for drug discovery against IE.
Keyphrases
- wild type
- biofilm formation
- escherichia coli
- pseudomonas aeruginosa
- oxidative stress
- staphylococcus aureus
- antimicrobial resistance
- drug discovery
- dna damage
- free survival
- candida albicans
- transcription factor
- ischemia reperfusion injury
- genome wide
- signaling pathway
- small molecule
- induced apoptosis
- human health
- protein protein
- drug induced
- genome wide identification