Molecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibility.
Inés Arrieta-AguirrePilar Menéndez-ManjónGiulia CarranoAnder DiezÍñigo Fernandez-de-LarrinoaMaria-Dolores MoraguesPublished in: Journal of fungi (Basel, Switzerland) (2023)
Vulvovaginal candidiasis (VVC) is a prevalent condition affecting women worldwide. This study aimed to develop a rapid qPCR assay for the accurate identification of VVC etiological agents and reduced azole susceptibility. One hundred and twenty nine vaginal samples from an outpatient clinic (Bilbao, Spain) were analyzed using culture-based methods and a multiplex qPCR targeting fungal species, which identified Candida albicans as the predominant species (94.2%). Antifungal susceptibility tests revealed reduced azole susceptibility in three (3.48%) isolates. Molecular analysis identified several mutations in genes associated with azole resistance as well as novel mutations in TAC1 and MRR1 genes. In conclusion, we developed a rapid multiplex qPCR assay that detects C. albicans in vulvovaginal specimens and reported new mutations in resistance-related genes that could contribute to azole resistance.
Keyphrases
- candida albicans
- high throughput
- biofilm formation
- genetic diversity
- bioinformatics analysis
- real time pcr
- primary care
- single cell
- pregnant women
- polycystic ovary syndrome
- high resolution
- escherichia coli
- cystic fibrosis
- gene expression
- cancer therapy
- skeletal muscle
- pregnancy outcomes
- fine needle aspiration
- ultrasound guided