Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels.
Gabriela Pacheco de Almeida BragaKarina Sampaio CaiaffaRafaela Laruzo RabeloVanessa Rodrigues Dos SantosAmanda Caselato Andolfatto SouzaLucas da Silva RibeiroEmerson Rodrigues de CamargoAnuradha PrakkiCristiane DuquePublished in: Journal of functional biomaterials (2023)
This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (CH) for evaluation of cell viability, total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition by colorimetric assays. Based on an initial screening, AMP and CH were loaded into PNVCL hydrogels and had their cytotoxicity and effect on mineralization markers determined. Cell viability was above 70% when MDPC-23 cells were treated with AMP, ISO and RUT. AMP showed the highest ALP activity and mineralized nodule deposition. Extracts of PNVCL+AMP and PNVCL+CH in culture medium (at the dilutions of 1/16 and 1/32) did not affect cell viability and stimulated ALP activity and mineralized nodules' deposition, which were statistically higher than the control in osteogenic medium. In conclusion, AMP and AMP-loaded PNVCL hydrogels were cytocompatible and able to induce bio-mineralization markers in odontoblast-cells.
Keyphrases
- protein kinase
- drug delivery
- induced apoptosis
- cell cycle arrest
- wound healing
- hyaluronic acid
- cancer therapy
- gold nanoparticles
- mesenchymal stem cells
- drug release
- cell death
- extracellular matrix
- oxidative stress
- high throughput
- room temperature
- bone regeneration
- risk assessment
- cell proliferation
- signaling pathway
- pi k akt
- aqueous solution