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Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus) - towards understanding gene function and regulatory evolution in an ecological model system for rapid phenotypic diversification.

Claudius F KratochwilMaggie M SeftonYipeng LiangAxel Meyer
Published in: BMC developmental biology (2017)
Transgenesis is a versatile tool not only for studying regulatory elements such as promoters and enhancers, but also for testing gene function through overexpression of allelic gene variants. As such, it is an important first step in establishing the Midas cichlid as a powerful model for studying adaptive coding and non-coding changes in an ecological and evolutionary context.
Keyphrases
  • risk assessment
  • human health
  • copy number
  • genome wide
  • transcription factor
  • genome wide identification
  • climate change
  • dna methylation
  • cell proliferation