Cytosporones with Anti-Inflammatory Activities from the Mangrove Endophytic Fungus Phomopsis sp. QYM-13.
Guisheng WangYilin YuanZhaokun LiJunhao ZhuZhi-Gang SheYan ChenPublished in: Marine drugs (2023)
Six previously undescribed cytosporone derivatives (phomotones A-E ( 1 - 5 ) and phomotone F ( 13 )), two new spiro-alkanol phombistenes A-B ( 14 - 15 ), and seven known analogs ( 6 - 12 ) were isolated from the mangrove endophytic fungus Phomopsis sp. QYM-13. The structures of these compounds were elucidated using spectroscopic data analysis, electronic circular dichroism (ECD), and 13 C NMR calculations. Compound 14 features an unprecedented 1,6-dioxaspiro[4.5]decane ring system. All isolates were evaluated for their inhibitory effect on nitric oxide (NO) in LPS-induced RAW264.7 cells. The results showed that compounds 1 , 6 , 8, and 11 exhibited potent bioactivities by comparing with positive control. Then, compound 1 displayed the anti-inflammatory effect by inhibiting the MAPK/NF- κ B signaling pathways. Molecular docking further revealed the possible mechanism of compound 1 interaction with ERK protein.
Keyphrases
- molecular docking
- signaling pathway
- lps induced
- anti inflammatory
- induced apoptosis
- pi k akt
- data analysis
- molecular dynamics simulations
- nitric oxide
- inflammatory response
- cell cycle arrest
- epithelial mesenchymal transition
- high resolution
- oxidative stress
- magnetic resonance
- molecular dynamics
- hydrogen peroxide
- immune response
- amino acid
- solid state
- structure activity relationship