A multicenter comparison of quantification methods for antisense oligonucleotide-induced DMD exon 51 skipping in Duchenne muscular dystrophy cell cultures.

Monika HillerMaria Sofia FalzaranoIker Garcia-JimenezValentina SardoneRuurd C VerheulLinda PopplewellKaren AnthonyEstibaliz Ruiz-Del-YerroHana OsmanJelle J GoemanKamel MamchaouiGeorge DicksonAlessandra FerliniFrancesco MuntoniAnnemieke Aartsma-RusVirginia Arechavala-GomezaNicole A DatsonPietro Spitali

Published in: PloS one (2018)

Our results suggest that digital droplet PCR was the most precise and quantitative method. The quantification of exon 51 skipping by Agilent bioanalyzer after a single round of PCR was the second-best choice with a 2.3-fold overestimation of exon 51 skipping levels compared to digital droplet PCR.

Keyphrases

duchenne muscular dystrophy
single cell
high throughput
real time pcr
muscular dystrophy
high glucose
diabetic rats
cell therapy
high resolution
oxidative stress
bone marrow