Detection of Rice Fungal Spores Based on Micro- Hyperspectral and Microfluidic Techniques.
Xiaodong ZhangHoujian SongYafei WangLian HuPei WangHanping MaoPublished in: Biosensors (2023)
As rice is one of the world's most important food crops, protecting it from fungal diseases is very important for agricultural production. At present, it is difficult to diagnose rice fungal diseases at an early stage using relevant technologies, and there are a lack of rapid detection methods. This study proposes a microfluidic chip-based method combined with microscopic hyperspectral detection of rice fungal disease spores. First, a microfluidic chip with a dual inlet and three-stage structure was designed to separate and enrich Magnaporthe grisea spores and Ustilaginoidea virens spores in air. Then, the microscopic hyperspectral instrument was used to collect the hyperspectral data of the fungal disease spores in the enrichment area, and the competitive adaptive reweighting algorithm (CARS) was used to screen the characteristic bands of the spectral data collected from the spores of the two fungal diseases. Finally, the support vector machine (SVM) and convolutional neural network (CNN) were used to build the full-band classification model and the CARS filtered characteristic wavelength classification model, respectively. The results showed that the actual enrichment efficiency of the microfluidic chip designed in this study on Magnaporthe grisea spores and Ustilaginoidea virens spores was 82.67% and 80.70%, respectively. In the established model, the CARS-CNN classification model is the best for the classification of Magnaporthe grisea spores and Ustilaginoidea virens spores, and its F1-core index can reach 0.960 and 0.949, respectively. This study can effectively isolate and enrich Magnaporthe grisea spores and Ustilaginoidea virens spores, providing new methods and ideas for early detection of rice fungal disease spores.
Keyphrases
- deep learning
- high throughput
- circulating tumor cells
- convolutional neural network
- machine learning
- early stage
- label free
- single cell
- loop mediated isothermal amplification
- squamous cell carcinoma
- electronic health record
- magnetic resonance imaging
- climate change
- real time pcr
- computed tomography
- optical coherence tomography
- locally advanced
- image quality