Ultrasound-Targeted Microbubble Destruction Mediates Gene Transfection for Beta-Cell Regeneration and Glucose Regulation.
Chao ZhangShuyuan ChenQunying LiJiao WuFuqiang QiuZhiyi ChenYang SunJieli LuoRaul A BastarracheaPaul A GrayburnRalph A DeFronzoYajing LiuKun QianPintong HuangPublished in: Small (Weinheim an der Bergstrasse, Germany) (2021)
Ultrasound-targeted microbubble destruction (UTMD) mediates gene transfection with high biosafety and thus has been promising toward treatment of type 1 diabetes. However, the potential application of UTMD in type 2 diabetes (T2D) is still limited, due to the lack of systematic design and dynamic monitoring. Herein, an efficient gene delivery system is constructed by plasmid deoxyribonucleic acid (DNA) encoding glucagon-like peptide 1 (GLP-1) in ultrasound-induced microbubbles, toward treatment of T2D in macaque. The as designed UTMD afforded enhancement of cell membrane penetration and GLP-1 expression in macaque, which is characterized by ultrasound-guided biopsy to monitor the dynamic process of islet cells for 6 months. Also, improvement of pancreatic beta cell regeneration, and regulation of plasma glucose in macaque with T2D is achieved. The approach would serve as promising alternatives for the treatment of T2D.
Keyphrases
- ultrasound guided
- type diabetes
- stem cells
- magnetic resonance imaging
- genome wide
- copy number
- cardiovascular disease
- single cell
- induced apoptosis
- bone marrow
- cell proliferation
- mesenchymal stem cells
- cancer therapy
- computed tomography
- adipose tissue
- climate change
- cell therapy
- endothelial cells
- gene expression
- genome wide identification
- transcription factor
- combination therapy
- blood glucose
- genome wide analysis
- single molecule
- long non coding rna
- signaling pathway
- diabetic rats
- glycemic control
- endoplasmic reticulum stress
- cell free
- wound healing