A TaqMan-based multiplex real-time PCR assay for the rapid detection of tigecycline resistance genes from bacteria, faeces and environmental samples.

Yiming LiZhangqi ShenShuangyang DingShaolin Wang

Published in: BMC microbiology (2020)

Taken together, the TaqMan-based real-time PCR method established in this study is rapid, sensitive, specific, and is capable of detecting the two genes not only in bacteria, but also in environmental samples.

Keyphrases

real time pcr
genome wide
bioinformatics analysis
genome wide identification
high throughput
life cycle
risk assessment
transcription factor
quantum dots
sensitive detection