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Dietary protein content and digestibility influences discrimination of amino acid nitrogen isotope values in a terrestrial omnivorous mammal.

John P WhitemanMauriel Rodriguez CurrasKelli L FeeserSeth D Newsome
Published in: Rapid communications in mass spectrometry : RCM (2021)
Diets were less digestible and included more protein in Experiment A than in Experiment B. As a result, the mice in Experiment A probably oxidized more AA, resulting in greater Δ15 NConsumer-Diet values. However, the similar responses of Δ15 NT-S and of TDFT-S to diet variation suggest that if diet samples are available, Δ15 NT-S accurately tracks trophic position. If diet samples are not available, the patterns presented here provide a basis to interpret Δ15 NT-S values. The trophic-source offset of Pro-Lys did not vary across diets, and therefore may be more reliable for omnivores than other offsets (e.g., Glu-Phe).
Keyphrases
  • weight loss
  • amino acid
  • physical activity
  • binding protein
  • high fat diet induced
  • gas chromatography
  • low density lipoprotein
  • tandem mass spectrometry