Porcine epidemic diarrhea virus (PEDV) is the pathogen of Porcine epidemic diarrhea (PED) and can mainly cause acute diarrhea, vomiting, dehydration and high mortality in neonatal piglets. The nucleocapsid (N) protein of PEDV is a highly conserved structural protein. In this study, 6-8-week-old BALB/c mice were immunized with purified PEDV, and three monoclonal antibodies (mAbs) against the PEDV N protein were generated, named 3C6,4F8,4C9. Among them, three new B cell epitopes, 235 IGENPDKL 242 , 12 KRVPLSLY 19 , 372 DAFKTGNA 380 were firstly identified in the viral N-protein. Among them, 4F8 and 4C9 had IgG1 isotype with Kappa light chain, while 3C6 had IgG2a isotype with Kappa light chain. Three monoclonal antibodies (mAbs) demonstrated specific reactivity with PEDV as evidenced by Western blot and indirect immunofluorescence assay. By studying the interaction between the mAbs and the N protein, we can gain insights into the protein's conformation and functional regions. This information will help develop fast and accurate PEDV diagnostic methods.
Keyphrases
- protein protein
- amino acid
- binding protein
- sars cov
- coronary artery disease
- clinical trial
- nuclear factor
- clostridium difficile
- high throughput
- high resolution
- intensive care unit
- south africa
- cardiovascular events
- metabolic syndrome
- social media
- insulin resistance
- respiratory syndrome coronavirus
- adipose tissue
- inflammatory response
- molecular dynamics simulations
- skeletal muscle
- respiratory failure