Reprogramming Postnatal Human Epidermal Keratinocytes Toward Functional Neural Crest Fates.
Vivek K BajpaiLaura KerosuoGeorgios TseropoulosKirstie A CummingsXiaoyan WangPedro LeiBiao LiuSong LiuGabriela K PopescuMarianne E BronnerStelios T AndreadisPublished in: Stem cells (Dayton, Ohio) (2017)
During development, neural crest (NC) cells are induced by signaling events at the neural plate border of all vertebrate embryos. Initially arising within the central nervous system, NC cells subsequently undergo an epithelial to mesenchymal transition to migrate into the periphery, where they differentiate into diverse cell types. Here we provide evidence that postnatal human epidermal keratinocytes (KC), in response to fibroblast growth factor 2 and insulin like growth factor 1 signals, can be reprogrammed toward a NC fate. Genome-wide transcriptome analyses show that keratinocyte-derived NC cells are similar to those derived from human embryonic stem cells. Moreover, they give rise in vitro and in vivo to NC derivatives such as peripheral neurons, melanocytes, Schwann cells and mesenchymal cells (osteocytes, chondrocytes, adipocytes, and smooth muscle cells). By demonstrating that human keratin-14+ KC can form NC cells, even from clones of single cells, our results have important implications in stem cell biology and regenerative medicine. Stem Cells 2017;35:1402-1415.
Keyphrases
- induced apoptosis
- stem cells
- cell cycle arrest
- endothelial cells
- genome wide
- endoplasmic reticulum stress
- oxidative stress
- gene expression
- single cell
- metabolic syndrome
- cell therapy
- cell proliferation
- type diabetes
- spinal cord
- spinal cord injury
- bone marrow
- dna methylation
- rna seq
- mesenchymal stem cells
- high fat diet induced