Chiral Discrimination of Penicillamine Enantiomers: The Role of Aggregation-Caused Quenching in Achieving High Selectivity.
Lin LanXiaoxue SongXuan KuangXu SunRui KuangPublished in: Analytical chemistry (2023)
The recognition and separation of chiral isomers are of great importance in both industrial and biological applications. In this study, a chiral recognition system based on electrochemiluminescence was established for the detection of penicillamine (PA) enantiomers. The system utilized a homochiral [Zn 2 (BDC)(d-lac)] (Zn-BL) platform for the uniform distribution of Ru(bpy) 3 2+ nanoparticles, effectively mitigating aggregation-caused quenching. The chiral recognition ability of Zn-BL was tested to distinguish between PA enantiomers, and the results indicated a substantial increase in the chiral electrochemiluminescence (ECL) signal when l-PA was present, in contrast to d-PA. The mechanism underlying ECL chiral discrimination was investigated using water contact angle measurements, DFT calculations, and electrochemical characterization. The system exhibited high selectivity, stability, and reproducibility for PA enantiomer detection. Furthermore, the proposed method can accurately identify one enantiomer of PA in a mixture. This study provides a reliable and sensitive approach for achieving the highly selective detection of chiral molecules.
Keyphrases
- capillary electrophoresis
- ionic liquid
- mass spectrometry
- heavy metals
- label free
- energy transfer
- loop mediated isothermal amplification
- sensitive detection
- real time pcr
- magnetic resonance imaging
- density functional theory
- magnetic resonance
- molecular dynamics
- risk assessment
- wastewater treatment
- single cell
- liquid chromatography
- contrast enhanced
- molecularly imprinted
- atomic force microscopy
- walled carbon nanotubes