cDNA Cloning and Partial Characterization of the DJ-1 Gene from Tribolium castaneum .
Shunya SasakiMaaya NishikoTakuma SakamotoMichael R KanostHiroko TabunokiPublished in: Antioxidants (Basel, Switzerland) (2021)
The DJ-1 gene is highly conserved across a wide variety of organisms and it plays a role in anti-oxidative stress mechanisms in cells. The red flour beetle, Tribolium castaneum , is widely used as a model insect species because it is easy to evaluate gene function in this species using RNA interference (RNAi). The T. castaneum DJ-1 ( TcDJ-1 ) sequence is annotated in the T. castaneum genome database; however, the function and characteristics of the TcDJ-1 gene have not been elucidated. Here, we investigated the cDNA sequence of TcDJ-1 and partially characterized its function. First, we examined the TcDJ-1 amino acid sequence and found that it was highly conserved with sequences from other species. TcDJ-1 mRNA expression was higher in the early pupal and adult developmental stages. We evaluated oxidant tolerance in TcDJ-1 knockdown adults using paraquat and found that adults with TcDJ-1 knockdown exhibited increased sensitivity to paraquat. Our findings show that TcDJ-1 has an antioxidant function, as observed for DJ-1 from other insects. Therefore, these results suggest that TcDJ-1 protects against oxidative stress during metamorphosis.
Keyphrases
- oxidative stress
- genome wide
- amino acid
- copy number
- induced apoptosis
- genome wide identification
- transcription factor
- dna damage
- ischemia reperfusion injury
- emergency department
- dna methylation
- gene expression
- mass spectrometry
- zika virus
- genome wide analysis
- cell death
- endoplasmic reticulum stress
- gram negative
- drug induced