NIR Light-Propelled Janus-Based Nanoplatform for Cytosolic-Fueled microRNA Imaging.
Fan LinYong ShaoYingjie WuYuanqing ZhangPublished in: ACS applied materials & interfaces (2021)
Various nanoplatforms have been developed to visualize intracellular microRNAs (miRNAs) because of their clinical significance in tumor progression and diagnosis. However, the diffusion-limited motion of the nanoplatforms penalizes the miRNA imaging efficiency in cells. Herein, we fabricated a near-infrared (NIR) light-propelled Janus-based nanoplatform to advance the imaging response. The Janus nanomotor covered with an Au half-shell was loaded by the endocytosis adjuvant of the MnO2 nanosheet for delivering a miRNA-responsive hQN (hairpin DNA quadrangular nanostructure) probe with a catalyzed hairpin assembly (CHA). Once the nanoplatform entered into cells, the MnO2 nanosheet was degraded to Mn2+ by endogenous fuels (such as glutathione) to release the hQN probe. The NIR light irradiation of the nanoplatform generated a heat gradient and thus propelled motion of the nanoplatform. This process accelerated the intracellular reaction of the hQN probe with miRNAs to trigger the cascade CHA amplification with an enhanced fluorescence readout.
Keyphrases
- photodynamic therapy
- drug release
- cancer therapy
- fluorescence imaging
- drug delivery
- induced apoptosis
- high resolution
- living cells
- fluorescent probe
- cell cycle arrest
- single molecule
- quantum dots
- early stage
- endoplasmic reticulum stress
- signaling pathway
- cell death
- room temperature
- oxidative stress
- cell proliferation
- sensitive detection
- radiation induced
- metal organic framework
- reduced graphene oxide