Pathogen quantitative efficacy of different spike-in internal controls and clinical application in central nervous system infection with metagenomic sequencing.
Zhangfan FuJingwen AiHaocheng ZhangPeng CuiTao XuYumeng ZhangYi ZhangHonglong WuAo ShenKe LinMiaoqu ZhangChao QiuNing JiangYang ZhouWenhong ZhangPublished in: Microbiology spectrum (2023)
phages, and artificial DNA sequence and developed the most applicable strategies for pathogen quantitation via mNGS in central nervous system infection. The IC application strategy we developed will enable mNGS analysis to assess the pathogen load simultaneously with the detection of pathogens, which should provide critical information for quick decision-making of treatment as well as clinical prognosis.
Keyphrases
- candida albicans
- decision making
- cerebrospinal fluid
- mass spectrometry
- single cell
- high resolution
- liquid chromatography tandem mass spectrometry
- healthcare
- cell free
- single molecule
- liquid chromatography
- gram negative
- loop mediated isothermal amplification
- health information
- combination therapy
- tandem mass spectrometry
- antibiotic resistance genes
- high performance liquid chromatography
- solid phase extraction
- circulating tumor cells