Use of Electron Paramagnetic Resonance (EPR) to Evaluate Redox Status in a Preclinical Model of Acute Lung Injury.
Hanan B ElajailiNathan M DeeSergey I DikalovJoseph P Y KaoEva S NozikPublished in: Molecular imaging and biology (2023)
We have developed protocols for delivering EPR spin probes in vivo, allowing detection of cellular and mitochondrial superoxide in lung injury by EPR. Superoxide measurements by EPR could differentiate mice with and without lung injury, as well as mouse strains with different disease susceptibilities. We expect these protocols to capture real-time superoxide production and enable evaluation of lung EPR imaging as a potential clinical tool for subphenotyping ARDS patients based on redox status.
Keyphrases
- hydrogen peroxide
- end stage renal disease
- newly diagnosed
- ejection fraction
- escherichia coli
- single molecule
- extracorporeal membrane oxygenation
- small molecule
- oxidative stress
- prognostic factors
- type diabetes
- acute respiratory distress syndrome
- fluorescence imaging
- risk assessment
- peritoneal dialysis
- lps induced
- density functional theory
- nitric oxide
- intensive care unit
- climate change
- living cells
- label free
- mesenchymal stem cells
- cell therapy
- adipose tissue