Trimodal Multiplexed Lateral Flow Test Strips Assisted with a Portable Microfluidic Centrifugation Device.
Man-Wen WangZong-Min ChenYung-Chun HsiehYi-Kai SuChun-Yi LinShun-Mao YangBor-Ran LiYang-Hsiang ChanPublished in: Analytical chemistry (2024)
During the COVID-19 pandemic, the use of lateral flow assays (LFAs) expanded significantly, offering testing beyond traditional health care. Their appeal lies in the ease of use, affordability, and quick results. However, LFAs often have lower sensitivity and specificity compared with ELISA and PCR tests. Efforts to improve LFAs have increased detection times and complexity, limiting their use in large-scale point-of-care settings. To address this, we propose a novel approach using probes that generate multiple signals to enhance the sensitivity and selectivity. This concept also allows multiplexed LFAs to detect multiple analytes concurrently. We developed a trimodal probe that integrates fluorescence, color, and magnetism into a single nanohybrid. The strong plasmonic absorption and high fluorescence of Au nanoparticles and polymer dots enable qualitative and semiquantitative diagnosis, while the magnetic signal facilitates accurate quantitative measurements. As proof-of-concept targets, we selected CYFRA 21-1 and CA15-3, biomarkers for lung and breast cancer, respectively. This trimodal LFA demonstrated a remarkable detection limit of 0.26 ng/mL for CYFRA 21-1 and 2.8 U/mL for CA15-3. To the best of our knowledge, this is the first platform of a trimodal LFA with multiplexing ability. The platform's accuracy and reliability were validated using clinical serum samples, showing excellent consistency with electrochemiluminescence immunoassay results. This universal concept can be applied to other targets, paving the way for the next-generation LFAs.
Keyphrases
- label free
- high throughput
- single molecule
- energy transfer
- healthcare
- sensitive detection
- single cell
- loop mediated isothermal amplification
- real time pcr
- living cells
- quantum dots
- high resolution
- molecularly imprinted
- small molecule
- reduced graphene oxide
- protein kinase
- structural basis
- circulating tumor cells
- mass spectrometry
- social media
- fluorescence imaging
- solid phase extraction
- walled carbon nanotubes