Genome-wide DNA methylation analysis of Metarhizium anisopliae during tick mimicked infection condition.

Nicolau SbarainiReinaldo BelliniAugusto Bartz PentericheRafael Lucas Muniz GuedesAne Wichine Acosta GarciaAlexandra Lehmkuhl GerberMarilene Henning VainsteinAna Tereza Ribeiro de VasconcelosAugusto SchrankCharley Christian Staats

Published in: BMC genomics (2019)

The set of genes here analyzed focused on secondary metabolites associated genes, known to be involved in several processes, including virulence. The BS-Seq pipeline and RT-qPCR analysis employing 5-Azacytidine led to identification of methylated virulence genes in M. anisopliae. The results provided evidences that DNA methylation in M. anisopliae comprises another layer of gene expression regulation, suggesting a main role of DNA methylation regulating putative virulence determinants during M. anisopliae infection cycle.

Keyphrases

genome wide
dna methylation
gene expression
escherichia coli
pseudomonas aeruginosa
staphylococcus aureus
biofilm formation
antimicrobial resistance
copy number
ms ms
candida albicans
rna seq