Slow pyrolysis, a widely recognized thermochemical technique, is employed to produce biochar usually under inert atmospheres. Recently, there is a growing interest in utilizing CO 2 as a carrier gas during pyrolysis as an alternative to inert atmospheres, aiming to modify the resulting pyrolytic products and make them suitable for different applications. This study investigated and compared the impact of CO 2 atmosphere with N 2 on pyrolysis of food waste, rice husk, and grape tree branches waste via slow pyrolysis at temperatures of 400, 500, and 600 °C at 5 and 15 °C/min for 1 h, to evaluate biochar production and its properties. The results demonstrate that CO 2 atmosphere increased the biochar yield for all feedstocks and significantly influenced the physicochemical properties of biochar. Compared to N 2 , CO 2 -derived biochar exhibited less volatile matter, higher carbon content, lower O/H and O/C molar ratios and enhanced textural properties. This study highlighted the potential of utilizing CO 2 for biochar production and tailoring biochar properties for specific applications and the findings contribute to the establishment of sustainable and efficient waste management systems and the production of value-added biochar products.