Improved Surface Functionalization and Characterization of Membrane-Targeted Semiconductor Voltage Nanosensors.
Joonhyuck ParkYung KuoJack LiYi-Lin HuangEvan W MillerShimon WeissPublished in: The journal of physical chemistry letters (2019)
Type-II ZnSe/CdS voltage-sensing seeded nanorods (vsNRs) were functionalized with α-helical peptides and zwitterionic-decorated lipoic acids (zw-LAs). Specific membrane targeting with high loading efficiency and minimal nonspecific binding was achieved. These vsNRs display quantum yield (QY) modulation as a function of membrane potential (MP) changes, as demonstrated at the ensemble level for (i) vesicles treated with valinomycin and (ii) wild-type HEK cells under alternating buffers with different [K+]. ΔF/F of ∼ 1% was achieved.
Keyphrases
- quantum dots
- wild type
- induced apoptosis
- cancer therapy
- reduced graphene oxide
- cell cycle arrest
- molecular dynamics
- signaling pathway
- energy transfer
- cell death
- machine learning
- convolutional neural network
- mass spectrometry
- room temperature
- oxidative stress
- molecularly imprinted
- highly efficient
- neural network
- visible light