Proximity ligation-transcription circuit-powered exponential amplifications for single-molecule monitoring of telomerase in human cells.
Li-Juan WangMeng-Meng LvJin-Ping HuMeng LiuShuangshuang ZhangPublished in: Chemical communications (Cambridge, England) (2023)
We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications. This strategy exhibits high sensitivity with a detection limit of 0.1 aM for the synthetic telomerase product TPC4 in vitro and 1 HeLa cell in vivo . Moreover, it can screen potential inhibitors, discriminate telomerase from interferents, and distinguish cancer cells from normal cells.
Keyphrases
- single molecule
- atomic force microscopy
- living cells
- cell cycle arrest
- induced apoptosis
- transcription factor
- papillary thyroid
- cell death
- high throughput
- cell therapy
- squamous cell
- mesenchymal stem cells
- squamous cell carcinoma
- oxidative stress
- endoplasmic reticulum stress
- bone marrow
- signaling pathway
- cell proliferation
- loop mediated isothermal amplification
- pi k akt
- childhood cancer
- high speed