A method for the determination of selected aromatic amino acid biomarkers of oxidative stress using microchip electrophoresis with electrochemical detection is described. The separation of the major reaction products of phenylalanine and tyrosine with reactive nitrogen and oxygen species was accomplished using ligand exchange micellar electrokinetic chromatography with a PDMS/glass hybrid chip. Electrochemical detection was achieved using a pyrolyzed photoresist film working electrode. The system was evaluated for the analysis of the products of the Fenton reaction with tyrosine and phenylalanine, and the reaction of peroxynitrite with tyrosine.
Keyphrases
- label free
- oxidative stress
- loop mediated isothermal amplification
- gold nanoparticles
- real time pcr
- molecularly imprinted
- amino acid
- dna damage
- liquid chromatography
- mass spectrometry
- wastewater treatment
- hydrogen peroxide
- capillary electrophoresis
- induced apoptosis
- high speed
- carbon nanotubes
- tandem mass spectrometry