Pre-analytical effects on whole transcriptome and targeted RNA sequencing analysis in cytology: The effects of prolonged time in storage of effusion specimens prior to preservation.
Gloria H SuraKevin TranChunxiao FuLili DuMichał MarczykRebekah E GouldEveline ChenAmy M TastoAgata Angelita TinnirelloW Fraser SymmansPublished in: Cytopathology : official journal of the British Society for Clinical Cytology (2023)
RNAseq can be accurately performed on effusion specimens after prolonged refrigerator storage. RNA extracted from scraped cytospin slides fixed in Carnoy's solution was marginally superior to 95% EtOH fixation, but either method had comparable analytic performance to high-quality FF RNA samples.