Single cell polarity in liquid phase facilitates tumour metastasis.
Anna LorentzenPaul F BeckerJan KoslaMassimo SainiKathrin WeidelePaolo RonchiCorinna KleinMonika J WolfFelix GeistBastian SeubertJane A McKeatingDaniela Mihic-ProbstKnud EsserMarko RoblekFelix KuehneGaia BiancoTracy O'ConnorQuentin MüllerKathleen SchuckSebastian LangeDaniel HartmannSaskia SpaichOlaf GroßJochen UtikalSebastian HaferkampMartin R SprickAmruta Damle-VartakAlexander HapfelmeierNorbert HüserUlrike ProtzerAndreas TrumppDieter SaurNachiket VartakChristoph A KleinBernhard PolzerLubor BorsigMathias HeikenwalderPublished in: Nature communications (2018)
Dynamic polarisation of tumour cells is essential for metastasis. While the role of polarisation during dedifferentiation and migration is well established, polarisation of metastasising tumour cells during phases of detachment has not been investigated. Here we identify and characterise a type of polarisation maintained by single cells in liquid phase termed single-cell (sc) polarity and investigate its role during metastasis. We demonstrate that sc polarity is an inherent feature of cells from different tumour entities that is observed in circulating tumour cells in patients. Functionally, we propose that the sc pole is directly involved in early attachment, thereby affecting adhesion, transmigration and metastasis. In vivo, the metastatic capacity of cell lines correlates with the extent of sc polarisation. By manipulating sc polarity regulators and by generic depolarisation, we show that sc polarity prior to migration affects transmigration and metastasis in vitro and in vivo.
Keyphrases
- induced apoptosis
- cell cycle arrest
- single cell
- end stage renal disease
- squamous cell carcinoma
- signaling pathway
- small cell lung cancer
- chronic kidney disease
- endoplasmic reticulum stress
- pseudomonas aeruginosa
- high throughput
- cell proliferation
- high resolution
- ejection fraction
- staphylococcus aureus
- prognostic factors
- ionic liquid
- peritoneal dialysis
- biofilm formation