A new mouse line for cell ablation by diphtheria toxin subunit A controlled by a Cre-dependent FLEx switch.
Nicholas W PlummerErica K UngewitterKathleen G SmithHumphrey H-C YaoPatricia JensenPublished in: Genesis (New York, N.Y. : 2000) (2017)
Recombinase responsive mouse lines expressing diphtheria toxin subunit A (DTA) are well established tools for targeted ablation of genetically defined cell populations. Here we describe a new knock-in allele at the Gt(Rosa)26Sor locus that retains the best features of previously described DTA alleles-including a CAG promoter, attenuated mutant DTA cDNA, and ubiquitous EGFP labeling-with the addition of a Cre-dependent FLEx switch for tight control of expression. The FLEx switch consists of two pairs of antiparallel lox sites requiring Cre-mediated recombination for inversion of the DTA to the proper orientation for transcription. We demonstrate its utility by Cre-dependent ablation of both a broad domain in the embryonic nervous system and a discrete population of cells in the fetal gonads. We conclude that this new DTA line is useful for targeted ablation of genetically-defined cell populations.
Keyphrases
- single cell
- escherichia coli
- cell therapy
- cancer therapy
- dna damage
- transcription factor
- gene expression
- dna methylation
- catheter ablation
- blood brain barrier
- stem cells
- magnetic resonance imaging
- computed tomography
- cell proliferation
- oxidative stress
- long non coding rna
- signaling pathway
- endoplasmic reticulum stress