Tumor mutational burden assessment in non-small-cell lung cancer samples: results from the TMB2 harmonization project comparing three NGS panels.
Javier Ramos-ParadasSusana Hernández-PrietoDavid LoraElena SanchezAranzazu RosadoTamara Caniego-CasasNuria CarrizoAna Belén EnguitaMaría Teresa Muñoz-JimenezBorja RodriguezUrbicio Perez-GonzalezDavid Gómez-SánchezIrene FerrerSantiago Ponce AixÁngel Nuñez BuizaPilar GarridoJosé PalaciosFernando Lopez-RiosEva Maria Garrido-MartinLuis Paz-AresPublished in: Journal for immunotherapy of cancer (2022)
Both panels exhibited robust analytical performances for TMB assessment, with stronger concordances in patients with negative PD-L1 expression. TSO500 showed a higher inter-laboratory reproducibility. The cut-offs for each assay were lowered to optimal overlap with FO.