Single-cell discovery of m 6 A RNA modifications in the hippocampus.
Shuangshuang FengMaitena Tellaetxe-AbeteYujie ZhangYan PengHan ZhouMingjie DongErika LarreaLiang XueLi ZhangMagdalena J KoziolPublished in: Genome research (2024)
N 6 -Methyladenosine (m 6 A) is a prevalent and highly regulated RNA modification essential for RNA metabolism and normal brain function. It is particularly important in the hippocampus, where m 6 A is implicated in neurogenesis and learning. Although extensively studied, its presence in specific cell types remains poorly understood. We investigated m 6 A in the hippocampus at a single-cell resolution, revealing a comprehensive landscape of m 6 A modifications within individual cells. Through our analysis, we uncovered transcripts exhibiting a dense m 6 A profile, notably linked to neurological disorders such as Alzheimer's disease. Our findings suggest a pivotal role of m 6 A-containing transcripts, particularly in the context of CAMK2A neurons. Overall, this work provides new insights into the molecular mechanisms underlying hippocampal physiology and lays the foundation for future studies investigating the dynamic nature of m 6 A RNA methylation in the healthy and diseased brain.
Keyphrases
- single cell
- cerebral ischemia
- rna seq
- subarachnoid hemorrhage
- high throughput
- blood brain barrier
- brain injury
- induced apoptosis
- nucleic acid
- cognitive impairment
- small molecule
- resting state
- spinal cord
- gene expression
- prefrontal cortex
- cell cycle arrest
- single molecule
- multiple sclerosis
- cell death
- spinal cord injury
- cognitive decline
- transcription factor
- cell proliferation
- current status
- cell therapy
- high resolution
- bone marrow
- mass spectrometry