Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches.

Wenjing LiuYourong GuoKaizhe WangXingfei ZhouYing WangJunhong LüZhifeng ShaoJun HuDaniel M CzajkowskyBin Li

Published in: Nanoscale (2019)

Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.

Keyphrases

single molecule
atomic force microscopy
living cells
circulating tumor
mass spectrometry
cell free