Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field.
Xuejia QianYuanzhao ShenJiasheng YuanChih-Tsung YangXin ZhouPublished in: Biosensors (2022)
Porcine epidemic diarrhea virus (PEDV), a coronavirus that causes highly infectious intestinal diarrhea in piglets, has led to severe economic losses worldwide. Rapid diagnosis and timely supervision are significant in the prophylaxis of PEDV. Herein, we proposed a gold-nanorod (GNR) probe-assisted counting method using dark field microscopy (DFM). The antibody-functionalized silicon chips were prepared to capture PEDV to form sandwich structures with GNR probes for imaging under DFM. Results show that our DFM-based assay for PEDV has a sensitivity of 23.80 copies/μL for simulated real samples, which is very close to that of qPCR in this study. This method of GNR probes combined with DFM for quantitative detection of PEDV not only has strong specificity, good repeatability, and a low detection limit, but it also can be implemented for rapid on-site detection of the pathogens.
Keyphrases
- loop mediated isothermal amplification
- label free
- high resolution
- living cells
- quantum dots
- real time pcr
- sars cov
- small molecule
- single molecule
- sensitive detection
- fluorescence imaging
- early onset
- photodynamic therapy
- fluorescent probe
- irritable bowel syndrome
- optical coherence tomography
- gram negative
- respiratory syndrome coronavirus