Broad-Spectrum Clearance of Lipopolysaccharides from Blood Based on a Hemocompatible Dihistidine Polymer.
Junjun ChenZhenqiang ShiXijing YangXiaoyu ZhangDongdong WangShengxu QianWenjing SunCunli WangQiongya LiZhengjian WangYanling SongGuangyan QingPublished in: ACS applied materials & interfaces (2023)
Blood infection can release toxic bacterial lipopolysaccharides (LPSs) into bloodstream, trigger a series of inflammatory reactions, and eventually lead to multiple organ dysfunction, irreversible shock, and even death, which seriously threatens human life and health. Herein, a functional block copolymer with excellent hemocompatibility is proposed to enable broad-spectrum clearance of LPSs from whole blood blindly before pathogen identification, facilitating timely rescue from sepsis. A dipeptide ligand of histidine-histidine (HH) was designed as the LPS binding unit, and poly[(trimethylamine N -oxide)- co -(histidine-histidine)], a functional block copolymer combining the LPS ligand of HH and a zwitterionic antifouling unit of trimethylamine N -oxide (TMAO), was then designed by reversible addition-fragmentation chain transfer (RAFT) polymerization. The functional polymer achieved effective clearance of LPSs from solutions and whole blood in a broad-spectrum manner and had good antifouling and anti-interference properties and hemocompatibility. The proposed functional dihistidine polymer provides a novel strategy for achieving broad-spectrum clearance of LPSs, with potential applications in clinical blood purification.
Keyphrases
- healthcare
- inflammatory response
- oxidative stress
- public health
- endothelial cells
- mental health
- intensive care unit
- acute kidney injury
- health information
- human health
- climate change
- candida albicans
- risk assessment
- multidrug resistant
- klebsiella pneumoniae
- drug release
- social media
- gram negative
- dna binding
- health promotion