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Targeting editing of tomato SPEECHLESS cis-regulatory regions generates plants with altered stomatal density in response to changing climate conditions.

Ido NirAlanta BudrysN Katherine SmootJoel ErberichDominique C Bergmann
Published in: bioRxiv : the preprint server for biology (2023)
Plants can change their shape, size and cellular composition in response to environmental cues. Here, by precise gene editing of a core stomatal development regulator gene in tomato, we generate new alleles with enhanced or dampened responses to light and temperature cues. Combined with live imaging of development, we show the genetic and cellular pathways that contribute to customization of the leaf epidermis, and how this could lead to better climate-adapted varieties.
Keyphrases
  • climate change
  • genome wide
  • crispr cas
  • transcription factor
  • copy number
  • high resolution
  • gene expression
  • risk assessment
  • genome wide identification
  • fluorescence imaging